Document Type : Original Article
Authors
1
Former M.Sc. Student of Biotechnology and Molecular Genetics of Horticultural Crops, Department of Horticultural Sciences, Faculty of Agriculture, Urmia University, Urmia
2
Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia
3
Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia
Abstract
Due to the problems raised from planting the seedlings in stone fruit orchards, the use of uniform and compatible rootstocks is essential. Tissue culture technique is one of the most important and useful methods for propagation of this type of rootstocks. In order to obtain suitable culture medium, type and concentration of sugar for proliferation of Gisela 6 under the in vitro conditions, present study was conducted in two separate experiments. In first experiment the effect of four culture media (MS, VS, QL and NN) was investigated in a completely randomized design. In the second experiment the type of sugar (sucrose, glucose, fructose and sorbitol) and different concentrations of sugar (15, 30 and 45 g.L-1) in a factorial experiment based on a completely randomized design with 5 replications were studied. Length and number of shoots per explant, number of nodes and leaves, fresh and dry weight of the explants and length of internodes were evaluated. According to the results, MS culture medium had the greatest effect on shoot length, and NN had the lowest effect on length and number of the shoots. Sorbitol (at all concentrations) had the lowest effect on proliferation rate of Gisela 6. The highest shoot length and number were observed in the concentration of 15 g.L-1 fructose and 45 g. L-1 glucose respectively. MS culture medium with 30 g.L-1 sucrose and glucose was the most suitable culture medium, for the proliferation of Gisela 6.
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