Evaluation of genetic diversity of Sistan native apple genotypes using IRAP and REMP markers

Document Type : Original Article

Authors

1 M.Sc. graduate in Medicinal Plants, Department of Horticulture and Landscape, Faculty of Agriculture, University of Zabol, Zabol, Iran.

2 Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.

3 Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.

4 Associate Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.

5 Assistant Professor, Department of Horticulture and Landscape, Faculty of Agriculture, University of Zabol, Zabol, Iran.

10.30466/rip.2021.53285.1155

Abstract

In order to study the genetic diversity of 25 apple genotypes from three regions of Hamoon, Benjar and Emamiyeh of Zabol city and one region of Zahedan, 11 primers of IRAP and 3 primers of REMP were used. All 14 primers used were able to identify a total of 61 polymorphic gene loci. According to the obtained results, the average percentage of polymorphism among the studied accessions was 39.79 %, with the lowest percentage of polymorphism belonging to the primer 83003 + K001 and the highest percentage of polymorphism attributed to the primer 8565. The content of polymorphic information (PIC) and marker index (MI) varied between 0.02 to 0.28 and 0.29 to 0.54 in all studied genotypes, respectively. The highest PIC was related to K006 primer, and the lowest PIC was related to K001 + 83003 primer, and the highest and lowest MI were also related to 8565 and K001 + 83003primers, respectively. The results strengthened the possibility that the retrotransposons, which produced the most polymorphisms, were more translocated in the evolution of the plants under study and replicated more copies within the genome. The results of cluster analysis based on the UPGMA method, 25 studied genotypes were categorized into three separate groups. Genotypes with geographical origin, as well as morphological similarities and the same horticultural traits, were placed in different groups, which could be due to the different amounts of mobile elements present in these genotypes.

Keywords

References

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Research in Pomology
Volume 7, Issue 1 - Serial Number 1
September 2022
Pages 19-28

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