Document Type : Original Article
M.Sc. of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran.
Professor, Department of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran.
Iranian Agricultural Biotechnology Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Qazvin, Iran.
Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Qazvin, Iran.
Date palm (Phoenix dactylifera L.) is a monocotyledonous, dioecious and perennial plant with a long life, which has high economic importance in Iran. Asexual embryogenesis is one of the most successful methods and a promising tool for mass propagation in commercial and ideal palm cultivars on a large scale.The tissue culture method of the date palm, including somatic embryogenesis, can lead to a somaclonal variation that is caused by genetic diversity and epigenetic variation. Therefore, the lack of genetic stability in tissue culture-derived plantlets leads to the unwillingness of the date palm orchardist to use this type of plantlets. In this research, the genetic stability of 4 maternal date palm and 180 in vitro seedlings obtained from somatyic embryogenesis was evaluated in the Medjool date cultivar using 24 pairs of SSR primers. Five control date cultivars Kabkab, Piyarom, Stamaran, Deiri and Barhi were used to screen of SSR primers that show polymorphism among cultivars. In results, none of the primers did succeed to reveal the differences among the tissue culture derived plants and its maternal plant cultivar. The results of this study showed that the date plants proliferated by tissue culture method genetically were similar to maternal plants. Therefore, the rapid and inexpensive method for propagation of the Medjool date palm to produce plants using in vitro somatic embryogenesis is recommended.