Document Type : Original Article
Authors
1
Former M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran.
2
Associate Professor, Horticulture Crops Research Department, Research and Educotion Center of West Azarbaijan, Agricultural and Natural Resources Research Center, AREEO, Urmia, Iran
3
Professor, Department of Plant Breeding and Biotechnology, Faculrty of Agriculture, Urmia Uiversity, Urmia, Iran.
Abstract
Developing new, seedless, early ripening and high quality cultivars are important goals in grape breeding programs. Nowadays, producing high percentage of seedless progeny has been possible. This study was aimed to examine the effects of ovule development stage (25, 35, 45, 55 and 65 days after pollination) and putrescine spraying (0, 20, 30 and 40 mg l-1) on size of the ovule and berry in grape Perletet cultivar during embryo rescue. Putrescine was applied at two different stages including 14 days before and 7 days after full bloom, respectively. After random collection of berries at above mentioned stages of ovule development, the ovules were removed and cultured on Nitsch and Nitsch medium containing 0.35 mg l-1 gibberllic acid and 1 mg l-1 indol acetic acid. Results showed that the effect of ovule developmental stage and putrescine pre-treating were significant on length and diameter of ovule and berry, as well as the percentage of ovule germination. The highest levels of studied traits were observed in 30 mg l-1 putrescine treatment at 65 days after pollination.
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